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当前位置: 首页 > 产品中心 > Cell_transfection > FormuMax/Trans Hi™ - 体外转染试剂/10 x 1.0 mL/F90101TH-101
商品详细FormuMax/Trans Hi™ - 体外转染试剂/10 x 1.0 mL/F90101TH-101
FormuMax/Trans Hi™ - 体外转染试剂/10 x 1.0 mL/F90101TH-101
FormuMax/Trans Hi™ - 体外转染试剂/10 x 1.0 mL/F90101TH-101
商品编号: F90101TH-101
品牌: liposomeexpert
市场价: ¥35980.00
美元价: 17090.50
产地: 美国(厂家直采)
公司:
产品分类: 细胞转染
公司分类: Cell_transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hiis very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.

Key Features for Trans-HiTM

  • Excellent transfection for single DNA transfection or multi DNA co-transfection
  • Rapid onset and long-lasting transfection
  • Suitable for very long DNAs
  • Simple and easy to use transfection procedure
  • Robust and reproducible transfection
  • High level of recombinant protein production
  • Very cost efficient
  • Long shelf-life: 12 months

Specifications:

Product type:In vitro cell transfection reagent
ApplicationsPlasmid transfection, protein overexpression
Technique:Polymer-based DNA nanoparticles
Cell type:Established cell lines, primary cells, hard-to-transfect cellsAdherent cells and suspension cells
Sample type:Plasmid DNA
Serum compatibility:Yes
Product size:0.5, 1.0 and 10x1.0 mL
Number of transfectionsUp to 660 in 24-well plates with 1.0mL reagentup to 330 transfections in 6-well plates
Shelf-life:12 months or longer when store refrigerated (2-8°C)
Shipping condition:Room temperature

Protocols

Trans-Hi™General Protocol For In Vitro Transfection in Mammalian Cells

Trans-Hi™ - Protocol for Transfecting 293 and CHO Suspension Cells

Trans-Hi™ - Protocol for Generation of rAAV

Trans-Hi™-Technical Tips For Good In Vitro Transfection

trans-hi-lipofectamine-compare.jpg

Comparison study ofIn vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine®2000in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per wellused for both products in 24 well plate 30 hours post transfection.

Trans-Hi™/DNA ratio (µL/µg) (HEK293T)

trans-hi-ratio.jpg

Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in24 well plate 30 hours post transfection, 0.5ug DNA per well).

hek293a-042116.png

Head-to-head comparisonTrans-Hi™ against Lipofectamine 2000HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).

Trans-Hi™Lipofectamine-2000
TimeDay 1Day 2Day 3Day 1Day 2Day 3
HEK293A (%)79.593.493.375.789.450.6

hela-042916c.jpg

Comparison study ofIn vitroplasmid DNA transfection of Trans-Hi™and Lipofectamine®2000in Hela cellswith GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well),24 well plate 48 hours post transfection).

Broad Transfection Spectrum for Mammalian Cell Types

Cell Lines

Efficiency (% GFP)

COS-7

85 - 90%

Hela

85 - 90%

HEK-293T

85-90%

NIH 3T3

75%

A549

75%

Hep3D

65-75%

SHEP

65-70%

CaCo2

60%

HepG2

70%

SKOV3

65%

MCF-7

65-70%

3T3-442A

35-40%

CHO

80-90%

品牌介绍
FormuMax科学公司位于加利福尼亚州北部硅谷的中心地带,是一家专业的药品配送公司,致力于满足医药/生物技术、化妆品和保健工业的配方开发外包的日益增长的需求。经营理念是成为**的伙伴,作为药物交付的***,特别是在制定具有挑战性的化合物脂质体和其他新的纳米技术。FormuMax拥有一支技术团队,在药物递送领域拥有***的专业知识,特别关注脂质体和其他基于脂质的技术。它们目前提供基础研究,配方可行性,定制配方准备,配方表征和分析科学的合同服务。FormuMax是**家提供大量预成型脂质体试剂的在线商店。已经成功地为来自世界各地的大学实验室、研究中心和各种行业的客户提供了服务和/或产品。