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FormuMax/100nm DOPC/CHOL Liposomes labeled with DiD/F60103F-DD/1 mL
FluorescentDOPC/CHOLliposomeslabeledwithDiD(100nm)
The fluorescentcontrolliposomes contain thelipophilicdyeDiDincorporatedinthebilayers.Itisusedfortrackingthecellularuptakeoftheliposomesbycommonmethodslikeconfocal,FACS,etc. Thefar-redfluorescent,lipophiliccarbocyanine DiD isalonger-wavelengthDiIanalog.Itisanoilatroomtemperatureandweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranes.Ithasanextremelyhighextinctioncoefficientandshortexcited-statelifetimes(~1nanosecond)inlipidenvironments.
Specifications
Lipidcomposition:DOPC/CHOL(54:45mol/mol)
Lipidconcentration:50mM(50-55mM)
Meanparticlesize: 100nm(90-120nm)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2
DiD: 0.5mM(0.48mg/mL)
StABIlity: 6monthsforunopenedvials
Storage:2-8 ºC
"DiD"; DiIC18(5)oil(1,1"-Dioctadecyl-3,3,3",3"-TetramethylindodicarbocyaninePerchlorate) (InvitrogenD-307)
Ex/Em: 644/665 nm
CASNumber: 127274-91-3
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL:cholesterol
