FluorescentDOPC/CHOLliposomeslabeledwithDiO(100nm)

The fluorescentcontrolliposomes contain thegreenlipophilicdyeDiOincorporatedinthebilayers. Itisusedfortrackingthecellularuptakeoftheliposomesbycommonmethodslikeconfocal,FACS,etc.  

The greenfluorescent,lipophiliccarbocyanine DiOC18(3) iswidelyusedasalipophilictracer.Itisweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranes.Ithasanextremelyhighextinctioncoefficientandshortexcited-statelifetimes(~1nanosecond)inlipidenvironments.

Specifications

Lipidcomposition:DOPC/CHOL(54:45mol/mol)

Lipidconcentration:50mM(50-55mM)

Meanparticlesize: 100nm(90-120nm)

Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2

Dye: 0.5mM(0.44mg/mL)

StABIlity: 6monthsforunopenedvials

Storage:2-8 ºC

'DiO';  DiOC18(3)(3,3'-DioctadecyloxacarbocyaninePerchlorate)  (InvitrogenD-275) 

Ex/Em: 484/501 nm

CASNumber: 34215-57-1

DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine

CHOL:cholesterol

rhodamineDHPE