FluorescentDOPC/CHOLliposomeslabeledwithDiR(100nm)
The fluorescentcontrolliposomes contain thelipophilicNearIRdyeDiRincorporatedinthebilayers.Itisusedfortrackingthecellularuptakeoftheliposomesinvitroandinvivobycommonmethodslikeconfocal,FACS,NIRimagingetc. ThenearIRfluorescent,lipophiliccarbocyanine DiOC18(7)("DiR") isweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranes.ThesulfonategroupsincorporatedintothisDiIanalogimproveswatersolubility.Ithasanextremelyhighextinctioncoefficientandshortexcited-statelifetimes(~1nanosecond)inlipidenvironments.
Specifications
Lipidcomposition:DOPC/CHOL(54:45mol/mol)
Lipidconcentration:50mM(50-55mM)
Meanparticlesize: 100nm(90-120nm)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2
DiR: 0.5mM(0.51mg/mL)
StABIlity: 6monthsforunopenedvials
Storage:2-8 ºC
"DiR"; (InvitrogenD-12731) DiIC18(7)(1,1"-Dioctadecyl-3,3,3",3"-TetramethylindotricarbocyanineIodide)
Ex/Em: 750/780 nm
CASNumber: n/a
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL:cholesterol
