FluorescentDOPC/CHOL/mPEG-DSPEliposomeslabeledwithOregonGreen®488DHPE(100nm)

HighqualityLUVliposomeslabeledwithOregonGreen®488DHPE(OG488-DHPE)arereadytouse. Theliposomesarepreparedbytheextrusionmethodtoabout100nmwithtightparticlesizedistribution. Theproductissterilefilteredandfilledinsterilevialsguaranteedtobestablefor6monthswhenstoredinrefrigerator.

Thephospholipid,OregonGreen®488DHPEislabeledontheheadgroupwiththegreen-fluorescentOregonGreen®fluorophorewithexcitation/emissionmaxima~496/524nm.Thisfluorinatedanalogoffluoresceinovercomessomeofthekeylimitationsoffluorescein,includinggreaterphotostABIlityandalowerpKa(pKa~4.7versus6.4forfluorescein),makingitsfluorescenceessentiallypHinsensitiveinthephysiologicalpHrange.

Specifications

Productcode:F60203F-OG

Lipidcomposition:DOPC/CHOL/mPEG-2000-DSPE/OG488-DHPE(49:45:5:1mol/mol)

Lipidconcentration:50mM(50-55mM)

OregonGreen488DHPE: 0.5mM(0.54mg/mL)

Meanparticlesize: 100nm(85-110nm)

Hydrationbuffer:10%sucrose,20mMHEPES,pH 7.3±0.2

Storage:in refrigerator(2-8degreeC)

Stability: 6monthforunopenedvials,protectfromlight 

DOPC:1,2-dioleoyl-sn-glycero-3-phosphocholine

CHOL:cholesterol

OregonGreen488DHPE:OregonGreen®4881,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (InvitrogenO-12650)