FluorescentDOPC/CHOL/mPEG-DSPEliposomeslabeledwithDil(100nm)
HighqualityLUVliposomeslabeledwithDilare readytouse.Theliposomesarepreparedbytheextrusionmethodto100nmwithverytightparticlesizedistribution. Theproductissterilefilteredandfilledinsterilevialsguaranteedtobestablefor6monthswhenstoredinrefrigerator.
Specifications
Lipidcomposition:DOPC/CHOL/mPEG2000-DSPE(50:45:5mol/mol)
Lipidconcentration:50mM(50-55mM)
DiI: 0.5mM(0.47mg/mL)
Meanparticlesize: 100nm(85-110nm)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2
Storage: in refrigerator(2-8degreeC)
Shelf-life: 6months guaranteedforunopenedvials
DiI;DiIC18(3) (InvitrogenV22889) isalipophilicmembranedye.Itisweaklyfluorescentuntilincorporatedintoliposomemembranes.Thisorange—red-fluorescentdye,whichisspectrallysimilartotetramethylrhodamine,isoftenusedasalong-termtracerforneuronalandothercells.
Ex/Em: 550nm/570nm
CASName/Number: 3H-Indolium,2-[3-(1,3-dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl1-octadecyl-,perchlorate/41085-99-8
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
mPEG2000-DSPE: 18:0PEG2000PE:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000](ammoniumsalt)
rhodamineDHPE
