FluorescentDOPC/CHOLliposomeslabeledwithDiO(100nm)
The fluorescentcontrolliposomes contain thegreenlipophilicdyeDiOincorporatedinthebilayers. Itisusedfortrackingthecellularuptakeoftheliposomesbycommonmethodslikeconfocal,FACS,etc.
The greenfluorescent,lipophiliccarbocyanine DiOC18(3) iswidelyusedasalipophilictracer.Itisweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranes.Ithasanextremelyhighextinctioncoefficientandshortexcited-statelifetimes(~1nanosecond)inlipidenvironments.
Specifications
Lipidcomposition:DOPC/CHOL/mPEG2000-DSPE(50:45;5mol/mol)
Lipidconcentration:50mM(50-55mM)
Meanparticlesize: 100nm(85-110nm)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2
Dye: 0.5mM(0.44mg/mL)
StABIlity: 6monthsforunopenedvials
Storage:2-8 ºC
'DiO'; DiOC18(3)(3,3'-DioctadecyloxacarbocyaninePerchlorate) (InvitrogenD-275)
Ex/Em: 484/501 nm
CASNumber: 34215-57-1
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
mPEG2000-DSPE: 18:0PEG2000PE:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000](ammoniumsalt)
rhodamineDHPE
