FluorescentDOPC/CHOL/mPEG-DSPEliposomeslabeledwithDiR(100nm)
The fluorescentcontrolliposomes contain thelipophilicNearIRdyeDiRincorporatedinthebilayers.Itisusedfortrackingthecellularuptakeoftheliposomesinvitroandinvivobycommonmethodslikeconfocal,FACS,NIRimagingetc. ThenearIRfluorescent,lipophiliccarbocyanine DiOC18(7)("DiR") isweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranes.ThesulfonategroupsincorporatedintothisDiIanalogimproveswatersolubility.Ithasanextremelyhighextinctioncoefficientandshortexcited-statelifetimes(~1nanosecond)inlipidenvironments.
Specifications
Lipidcomposition:DOPC/CHOL/mPEG2000-DSPE(50:45:5mol/mol)
Lipidconcentration:50mM(50-55mM)
Meanparticlesize: 100nm(85-110nm)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2
DiR: 0.5mM(0.51mg/mL)
StABIlity: 6monthsforunopenedvials
Storage:2-8 ºC
"DiR"; (InvitrogenD-12731) DiIC18(7)(1,1"-Dioctadecyl-3,3,3",3"-TetramethylindotricarbocyanineIodide)
Ex/Em: 750/780 nm
CASNumber: n/a
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL:cholesterol
mPEG2000-DSPE: 18:0PEG2000PE:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000](ammoniumsalt)
