FluorescentDOPC/CHOL/mPEG-DSPEliposomeslabeledwithDiD(100nm)
The fluorescentcontrolliposomes contain thelipophilicdyeDiDincorporatedinthebilayers.Itisusedfortrackingthecellularuptakeoftheliposomesbycommonmethodslikeconfocal,FACS,etc. Thefar-redfluorescent,lipophiliccarbocyanine DiD isalonger-wavelengthDiIanalog.Itisanoilatroomtemperatureandweaklyfluorescentinwaterbuthighlyfluorescentandquitephotostablewhenincorporatedintomembranes.Ithasanextremelyhighextinctioncoefficientandshortexcited-statelifetimes(~1nanosecond)inlipidenvironments.
Specifications
Lipidcomposition: DOPC/CHOL/mPEG-2000-DSPE(50:45:5mol/mol)
Lipidconcentration:50mM(50-55mM)
Lipidconcentration:50mM(50-55mM)
Meanparticlesize: 100nm(85-110nm)
Hydrationbuffer: 10%sucrose,20mMHEPES,pH 7.3±0.2
DiD: 0.5mM(0.48mg/mL)
StABIlity: 6monthsforunopenedvials
Storage:2-8 ºC
"DiD"; DiIC18(5)oil(1,1"-Dioctadecyl-3,3,3",3"-TetramethylindodicarbocyaninePerchlorate) (InvitrogenD-307)
Ex/Em: 644/665 nm
CASNumber: 127274-91-3
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL:cholesterol
mPEG2000-DSPE: 18:0PEG2000PE:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000](ammoniumsalt)
