FluorescentDOPC/CHOLliposomeslabeledwithOregonGreen®488DHPE(100nm)

HighqualityLUVliposomeslabeledwithOregonGreen®488DHPE(OG-DHPE)arereadytouse.Theliposomesarepreparedbytheextrusionmethodto100nmwithverytightparticlesizedistribution.Theproductissterilefilteredandfilledinsterilevialsguaranteedtobestablefor6monthswhenstoredinrefrigerator.Theproductissterilefilteredandfilledinsterilevialsguaranteedtobestablefor3monthswhenstoredinrefrigerator.

Thephospholipid,OregonGreen®488DHPEislabeledontheheadgroupwiththegreen-fluorescentOregonGreen®fluorophorewithexcitation/emissionmaxima~496/524nm.Thisfluorinatedanalogoffluoresceinovercomessomeofthekeylimitationsoffluorescein,includinggreaterphotostABIlityandalowerpKa(pKa~4.7versus6.4forfluorescein),makingitsfluorescenceessentiallypHinsensitiveinthephysiologicalpHrange.

Specifications

Productcode:F60103F-OG

Lipidcomposition:DOPC/CHOL/OG-DHPE(54:45:1mol/mol)

Lipidconcentration:50mM(50-55mM)

OregonGreen488DHPE:0.5mM(0.54mg/mL)

Meanparticlesize:100nm(90-120)

Hydrationbuffer:10%sucrose,20mMHEPES,pH7.3±0.2

Storage:inrefrigerator(2-8degreeC)

Stability:6monthforunopenedvials,protectfromlight

DOPC:1,2-dioleoyl-sn-glycero-3-phosphocholine

CHOL:cholesterol

OregonGreen488DHPE:OregonGreen®4881,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine(InvitrogenO-12650)